alkA [2018-05-20 18:21:06]

DNA-3-methyladenine glycosylase

Locus: BSU01800

Isoelectric point: 8.15

Molecular weight: 35.48 kDa

Protein length: 303 aa

Gene length: 909 bp

Function: adaptive response to alkylative DNA damage

Product: DNA-3-methyladenine glycosylase

Essential: no

E.C.: 3.2.2.21

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins
• Prophages and mobile genetic elements → Prophages → Prophage 1

Gene

Coordinates: 202,547 → 203,458

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to Swiss-Prot)

Protein family

• alkylbase DNA glycosidase alkA family (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: alkA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AdaA: positive regulation, in AdaA regulon

Biological materials

Mutant

• BKE01800 (ΔalkA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTTTCATTCTCCTTAT, downstream forward: _UP4_TGAATCATTTCAACTTTAAC
• BKK01800 (ΔalkA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTTTCATTCTCCTTAT, downstream forward: _UP4_TGAATCATTTCAACTTTAAC

References

Reviews

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Original publications

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